Chi-How Peng| National Tsing Hua University | Hsinchu, Taiwan
Gene therapy based on small interference RNA (siRNA) is a promising strategy against multi-drug resistance in cancer treatment. However, siRNA needs a carrier to avoid the degradation caused by enzymes in circulation. In this report, a triblock copolymer of poly(ethylene glycol)-b-poly (2-dimethylaminoethyl methacrylate)-b-poly(pyrenylmethyl methacrylate) (PEG-b-PDMAEMA-b-PPy) was used to prepare a delivery system for siRNA, which is responsive to the change of both pH and UV light. The carrier was demonstrated to be stable under physiological condition. In contrast, up to 91% of siRNA could be released within 24 hours with the irradiation of UV light under a low-pH environment. The in vitro test showed that the knockdown efficiency of the siRNA-condensed micelleplexes was 50%, demonstrating the potential of these micelleplexes on stimuli-responsive gene therapy.