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EACON 2020

EurAsian Conference on Nanophotonics

April 6 – 9, 2020 / Jena, Germany

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Po-Chiao Lin

Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan

New Environment Sensitive Fluorophores with Color-Tailored Emission: In Vivo Monitoring of Carbonic Anhydrases Expression on Growth of Larval Zebrafish

Protein expression in space and time is significant for understanding important biological processes. This study monitors dynamic progress of newly developed background-free, target responsive strategy; a fluorophore 2,3-dihydroquinolin-4-imine (DQI) that can instantly respond to environmental changes with fluorescence enhancement, revealing a comprehensive platform for in vivo fluorescence bioimaging of target proteins. The density functional theory (DFT) calculation rationalized this response showing considerable change of a dipolar moment between excited and ground states. The novel environment-sensitive property makes DQI an ideal fluorescent turn-on biosensor for continuous and selective biomolecular recognition. An arylsulfonamide-conjugated DQI (SA-DQI) molecule successfully detected human carbonic anhydrase II (hCAII) and SA-DQI can recognize membrane-bound hCAII on HeLa cells without removal of unbound SA-DQI. This chemical strategy allows further instantaneous monitoring of carbonic anhydrase (CA) expression responding to the formation of otolith during zebrafish embryogenesis a promising advancement for study of protein dynamic expression upon chemical and biological stimuli.

References

  1. C.-H. Chou, B. Rajagopal, C.-F. Liang, K.-L. Chen, D.-Y. Jin, H.-Y. Chen, H.-C. Tu, Y.-Y. Shen, P.-C. Lin Chem. Eur. J., 2018, 24, 1112–1120.
  2. S.-C. Tang, H. Ma, H.-C. Tu, H.-R. Wang, P.-C. Lin* and Kristi S. Anseth* Adv. Science 2018, 1800638.
  3. K.-K. Yu, W.-B. Tseng, M.-J. Wu, A. S. K. K. Alagarsamy, W.-L. Tseng, P.-C. Lin Sens. actuators. B Chem. 2018, 273, 681–688.
  4. C.-H. Chou, P.-C. Lin* Biomacromolecules, 2018, 19, 3086–3095.

Further Talks

Andrea Csáki

Microfludic Synthesis of plasmonic Nanoparticles for Sensing Applications

Andrey Turchanin

Controlled growth of transition metal dichalcogenide monolayers for applications in nanoelectronic and nanophotonic devices

Chi Chen

Near Field Spectroscopic Imaging: from Hard to Soft Materials

Chi-How Peng

Cobalt mediated controlled/living radical polymerization: from mechanism understanding to materials application

Chun-Hong Kuo

Bridging Energy and Chemistry via Nanoarchitectonic Engineering at Atomic Scale

Falk Eilenberger

Integration of two-dimensional materials in optical systems for nonlinear optics, sensing, and single photon sources

Frank Setzpfandt

Nonlinear frequency conversion in nanostructured optical systems for application in quantum photonics

Heng-Liang Wu

Controlling the Oxidation State of Cu Electrode and Reaction Intermediates for Electrochemical CO2 Reduction to Ethylene

Kien-Voon Kong

Direct Observation of Reaction Intermediates of Metal-based Therapeutic Agents in Single Living Cell Using TERS

Maria Wächtler

Quantum confined semiconductor nanostructures in light-driven catalysis

Martin Presselt

Supramolecular Control of Optical and Electronical Properties of Two-Dimensional Dye Layers

Po-Chiao Lin

New Environment Sensitive Fluorophores with Color-Tailored Emission: In Vivo Monitoring of Carbonic Anhydrases Expression on Growth of Larval Zebrafish

Shangjr (Felix) Gwo

Plasmonic Metasurface-Enhanced Linear and Nonlinear Processes in Two-Dimensional Semiconductors

Ta-Jen Yen

Empowering Bilayer MoS2 by Engineered Plasmonic Nanostructures for Optoelectronic Applications

Tiow-Gan Ong

Carbodicarbene, Carbogenic Maverick, Not a Moderate!

Volker Deckert

Plasmon Enhanced Probe Spectroscopies – Structural Investigation of Nanoscale Objects

Yi-Tsu Chan

Molecular self-assembly methodology for rational construction of metallosupramolecules with high structural complexity

Yian Tai

Toward a universal polymeric material for electrode buffer layers in organic optoelectronics

Yu-Chieh Cheng

Kirigami-based soft robots with light-responsive properties

Yu-Jung Lu

Controlling Light-matter Interactions at Nanoscale with Plasmonics: From Spontaneous Emission to Lasing